Journal: Cell Death & Disease

Article Title: Intrinsic regulation of hemangioma involution by platelet-derived growth factor

doi: 10.1038/cddis.2012.58

Figure Lengend Snippet: PDGF-BB inhibits adipogenic differentiation in hemSCs. ( a ) Representative oil red O staining of hemSCs cultured in adipogenic differentiation media supplemented with 10 ng/ml of PDGF-AA, -AB, or -BB. ( b ) Quantitative analysis of adipogenic differentiation in the presence of PDGF polypeptides was assessed by real-time RT-PCR for adipogenesis-specific transcription factors, C/EBP α and PPAR γ (data normalized to 18 S rRNA and presented as relative to adipogenic media; * P <0.05 compared with the adipogenic media; n =3). ( c ) Effect of adipogenic differentiation on PDGFR expression in hemSCs at day 7. ( d ) Oil red O staining of hemSCs cultured with different growth factors (each at 10 ng/ml) illustrating specific inhibition of hemSC adipogenesis by PDGF signaling

Article Snippet: The expression of FABP-4 is developmentally regulated by fatty acids and is expressed by mature adipocytes., To study the effect of PDGF on hemSC adipogenic differentiation, we included 10 ng/ml PDGF-AA, PDGF-AB, PDGF-BB, SCF, SDF-1, or VEGF-A and -B in the adipogenic differentiation media (all growth factors were obtained from R & D Systems: 221-AA-010, 20-BB-010, 222-AB-010, 255-SC-010, 350-NS-010, 293-VE-010, and 751-VE-025).

Techniques: Staining, Cell Culture, Quantitative RT-PCR, Expressing, Inhibition

Journal: Cell Death & Disease

Article Title: Intrinsic regulation of hemangioma involution by platelet-derived growth factor

doi: 10.1038/cddis.2012.58

Figure Lengend Snippet: PDGF-BB, but no other PDGF polypeptides, prevent full functional differentiation of hemSCs. ( a ) Oil red O staining of hemSCs cultured in low (10 ng/ml) and high (50 ng/ml) PDGF polypeptide levels. PDGF-AA and –AB were ineffective against preventing adipogenic differentiation at 10 and 50 ng/ml. ( b ) Quantitative analysis of early adipogenesis-specific transcription factors (C/EBP α and PPAR γ ) and late marker of adipocytes, FABP-4 (* P <0.05 compared to adipogenic media; n =3). ( c ) Expression of FABP-4 in hemSCs treated with 50 ng/ml PDGF-AA. ( d ) Inhibition of adipogenesis by PDGF-BB in bm-MPCs (data presented relative to adipogenic media; * P <0.05 compared with adipogenic media; n =3)

Article Snippet: The expression of FABP-4 is developmentally regulated by fatty acids and is expressed by mature adipocytes., To study the effect of PDGF on hemSC adipogenic differentiation, we included 10 ng/ml PDGF-AA, PDGF-AB, PDGF-BB, SCF, SDF-1, or VEGF-A and -B in the adipogenic differentiation media (all growth factors were obtained from R & D Systems: 221-AA-010, 20-BB-010, 222-AB-010, 255-SC-010, 350-NS-010, 293-VE-010, and 751-VE-025).

Techniques: Functional Assay, Staining, Cell Culture, Marker, Expressing, Inhibition

Journal: Cell Death & Disease

Article Title: Intrinsic regulation of hemangioma involution by platelet-derived growth factor

doi: 10.1038/cddis.2012.58

Figure Lengend Snippet: Inhibiting cell-autogenous PDGF signaling enhances adipogenesis. ( a ) Oil red O staining of hemSCs exposed to adipogenic media with or without PDGFR inhibitors, AG-370 and AG-1296, and PDGFR neutralizing antibodies. ( b ) Quantitative analysis of C/EBP α and PPAR γ in cells exposed to PDGF inhibitors as in ( a ) (data presented relative to control media; * P <0.05 compared with control media; † P <0.05 compared with adipogenic media; n =3). ( c ) Effect of PDGFR- α and PDGFR- β- neutralizing antibodies and chemical inhibitors on reversing the inhibition by exogenous PDGF-BB (* P <0.05 compared with PDGF-BB treatment)

Article Snippet: The expression of FABP-4 is developmentally regulated by fatty acids and is expressed by mature adipocytes., To study the effect of PDGF on hemSC adipogenic differentiation, we included 10 ng/ml PDGF-AA, PDGF-AB, PDGF-BB, SCF, SDF-1, or VEGF-A and -B in the adipogenic differentiation media (all growth factors were obtained from R & D Systems: 221-AA-010, 20-BB-010, 222-AB-010, 255-SC-010, 350-NS-010, 293-VE-010, and 751-VE-025).

Techniques: Staining, Control, Inhibition

Journal: Cell stem cell

Article Title: The Mitochondrial Transacylase, Tafazzin, Regulates AML Stemness by Modulating Intracellular Levels of Phospholipids

doi: 10.1016/j.stem.2019.02.020

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: autoMACs Rinsing Solution , MACS Miltenyi Biotec , Cat# 130-091-222.

Techniques: Recombinant, Modification, Western Blot, Binding Assay, XF Assay, Electron Microscopy, Staining, High Performance Thin Layer Chromatography, Reverse Transcription, Plasmid Preparation, Apoptosis Assay, DC Protein Assay, SYBR Green Assay, Gene Expression, shRNA, Control, CRISPR, Software